A Study of Potassium Permanganate ' Fixation ' for Electron Microscopy
نویسنده
چکیده
The action of buffered potassium permanganate as a fixative for electron microscopy has been investigated. Electron contrast has been shown to be produced by the deposition of granular material in the tissue. The particles are about 50 A in diameter. Actual fixation of the tissue is performed by the dehydrating alcohol. Histochemical studies have shown that RNA and histones are removed, whereas phospholipid-protein complexes are 'unmasked'. The reaction of the permanganate with unmasked protein gives rise to high membrane contrast in electron micrographs.
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